Identification of mast cells in the brain of mice with lipopolysaccharide-induced neuroinflammation

DOI: https://doi.org/10.29296/24999490-2023-06-09

K.V. Ichetkina, A. Ismailova, O.P. Tuchina
Federal State Autonomous Educational Institution of Higher Education "BFU named after Immanuel Kant", Educational
and Scientific Cluster "Institute of Medicine and Life Sciences (MEDBIO)", Higher School of Living Systems, Laboratory of Synthetic Biology,
Russian Federation, 236041, Kaliningrad, st. Universitetskaya, 2

The purpose of the present study was to identify mast cells in brain sections of mice with neuroinflammation induced by intraperitoneal injections of LPS. Material and methods. The subjects of the study were nonlinear mice that received intraperitoneal injections of LPS at a dose of 1 mg/kg or sterile 0.9% NaCl. Serial frontal sections of the brain were prepared using a cryostat. To identify mast cells, sections were stained with brilliant green and methylene blue. Results. The findings suggest that intraperitoneal administration of LPS increases the number of resident mast cells in the mouse brain, especially in the perivascular areas between the hippocampus and thalamus. The increase in the number of cells appears to be the result of their division or directed migration under conditions of developing inflammation. Conclusion. Administration of LPS leads to an increase in the number of mast cells in the brain of mice. Mast cells are likely to be an important source of immune mediators in LPS-induced neuroinflammation, as evidenced by their active degranulation process.
Keywords: 
mast cells, hippocampus, lipopolysaccharide, cytokines, neuroinflammation
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