PROLIFEROTROPIC EFFECT OF COMBINATIONS OF SHORT PEPTIDES AND ENCODED AMINO ACIDS IN ORGANOTYPIC CULTURE OF TISSUE OF DIFFERENT GENESIS

DOI: https://doi.org/10.29296/24999490-2023-05-07

N.I. Chalisova(1, 2), P.N. Ivanova(1), E.S. Egozova(3)
1-nstitute of Physiology named after. I.P. Pavlova RAS, emb. Makarova, 6, St. Petersburg, 199034, Russian Federation;
2-St. Petersburg Institute of Bioregulation and Gerontology, Dynamo Ave., 3, St. Petersburg, 197110, Russian Federation;
3-Russian State Pedagogical University named after. A.I. Herzen, emb. R. Moiki, 48, St. Petersburg, 191186, Russian Federation

Introduction. An urgent problem in biology and medicine is the identification of biologically active molecules that affect the cellular processes of proliferation and apoptosis in various tissues of the body. Purpose of the study. The purpose of this study was to identify the effect of combinations of encoded amino acids and short peptides in organotypic tissue culture of various genesis of young (3-month-old) and old (18-month-old) rats. Method. The method of organotypic cultivation of tissues of mesodermal origin (cartilage, kidney, testes) and ectodermal origin (pancreas) of rats was used for rapid screening of the biological activity of the studied substances. Results. It has been established that the combined use of amino acids and short peptides enhances their proliferative effect, which leads to a potentiating effect of the drugs used. Conclusion. The data obtained create the basis for the targeted development of new drugs, including geroprotective ones, for the treatment of pathologies of the nervous and immune systems.
Keywords: 
proliferation, encoded amino acids, short peptides, organotypic tissue culture
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