CLINICAL AND MICROSCOPIC ANALYSIS OF CELLULAR-MOLECULAR COMMUNICATIONS IN FOCAL CORTICAL DYSPLASIA IIIс

DOI: https://doi.org/10.29296/24999490-2023-03-06

L.B. Mitrofanova(1), Z.M. Rasulov(1), O.M. Vorobieva(1), A.N. Gorshkov(1, 2), K.A. Sterkhova(1), A.Yu. Ulitin(1),
1-FSBI «NMIC named after V.A. Almazov», Akkuratova str., 2, St. Petersburg, 197341, Russian Federation;
2-FGBU «A.A. Smorodintsev Influenza Research Institute», Prof. Popov str., 15/17, St. Petersburg, 197022, Russian Federation

Cortical dyslamination with neuronal dysmorphism that occurs adjacent to an arteriovenous malformation (AVM) and is accompanied by epilepsy (E) is classified as FCD IIIc. Its etiology and pathogenesis have yet to be determined. Objective: to clarify the cellular composition and expression of various receptors in the AVM and its perifocal zone with and without FCD IIIc Material and methods. A morphological study of the surgical material of the brain of 14 patients with FCD IIIc and 13 patients with AVM without E was carried out using antibodies to: Ang1, Ang2, Ki-67, MHC1, CD34, NeuroD1, NG2, CD117, PrgRc, ErgRc, SSTR2, GH, SMA, GFAP and electron microscopy of the AVM of 1 patient with FCD IIIc. Results. There were CD34+ endotheliocytes, CD34+/CD117+/NeuroD1+ telocytes, SMA+ smooth muscle cells, NG2+ pericytes in the walls of AVM vessels with E and without it. A scar zone of CD117+-telocytes forming a 3D structure was determined in 50% of patients with FCD IIIc and in 46% with AVMs. Electron microscopy confirmed the presence of pericytes and telocytes in the small AVM vessels. In no case was the expression of PrgRc, ErgRc, and GH, while SSTR2 was detected in vascular cells of all AVMs and the perifocal zone. The expression level of MHC1 was statistically significantly higher in the AVM vessels than around the vessels (p
Keywords: 
arteriovenous malformation, epilepsy, telocytes and pericytes, somatostatin receptors
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