COMBINED EFFECT OF POLYPEPTIDE COMPLEXES ON THE DEVELOPMENT OF ORGANOTYPIC CULTURE OF RAT TISSUES

DOI: https://doi.org/10.29296/24999490-2023-01-09

N.I. Chalisova(1, 3), G.A. Ryzhak(3), E.S. Egorova(2)
1-I.P. Pavlov Institute of Physiology of the Russian Academy of Sciences, nab. Makarova, 6, St. Petersburg, 199034, Russian Federation;
2-A.I. Herzen Russian State Pedagogical University, Moika River Embankment, 48, Saint Petersburg, 191186, Russian Federation;
3-St. Petersburg Institute of Bioregulation and Gerontology, Аve. Dynamo, 3, St. Petersburg, 197110, Russia

Introduction. The formation of effective bioregulators for the maintenance of the basic physiological functions, so as polypeptide complexes (PPCs), is one of the actual task of modern biology and medicine. Purpose of study. Effect of combination of polypeptide complexes (PPCs) from thymus (Thymalin) with PPC from liver (Ventvil), PPC from kidney (Nephropept) and PPC from brain, (Сortexin) on the cellular proliferation of rat corresponding tissues. Methods. Organotypic tissue culture of rat spleen, liver, kidney and brain cortex under different PPCs and their combinations effect. Results. All PPCs increase statistically reliable the square index (SI) of rat corresponding tissues explants of young rats skin explants on 20–27%, as compared to the control. There was no potentiate effect and the stimulation of the cellular proliferation was at the account of this PPC, which stimulated the proliferation by its single effect. Conclusion. The obtained data create a basis for using by the patient combined pathology of two different PPCs, each of them will have effect on the regeneration stimulation only of corresponding tissue.
Keywords: 
polypeptide complexes, organotypic tissue culture, combined pathology
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